ABSTRACT
Objective: The objective of this study was to evaluate the cytotoxic potential of A3AR agonist (ABMECA) against human lung cancer cell line A549 by using 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT) assay. Methods: Adenocarcinoma cell line A549 was used to assess MTT based cells viability. In vitro cytotoxic activity was evaluated for 3 different concentration of doxorubicin and A3AR by MTT cytotoxicity assay. Cytotoxicity assay carried out for 3 consecutive days that involves culturing cells into Dulbecco’s MEM medium modified with 10% FBS for 24 h then treatment with different dose of standard and test drug with incubation period of 24 h followed by treatment with MTT for estimation of cytotoxicity and finally, optical density (OD) was measured at 570-630 nm. Results: Different concentration of doxorubicin (1, 5, 10 µM) and ABMECA (10-6M, 10-5M and 10-4M) shown dose-dependent cytotoxicity. There was a significant decrease (p<0.05) in cell viability in both doxorubicin and ABMECA concentration in a dose-dependent manner. This study may guide further for in vivo evaluation of test drug in the lung cancer model. Conclusion: A3 Adenosine Receptor agonist could be potential moiety for the treatment of lung cancer and it would require in vivo study for further research.
ABSTRACT
Randia dumetorum family Rubiaceae is highly reputed ayurvedic medicinal tree commonly known as the Mainphal, Mindhal. A large deciduous thorny shrub grows up to 5 meters of height. It occurs in almost throughout India up to 4,000 ft attitude. It is found in Himalaya from jammu east words ascending to 1300 and from the Kashmir to east word’s up to 4000. It is seen in Gujarat, Tamilnadu forest of Dehradun, Suralik range, Bengal, Bihar, Orrisa & South Maharashtra and costal districts of south India. In dry deciduous forests in India. Root bark of Randia dumetorum contains triterpene, -1-keto-3-hydroxyoleanane, Bark of Randia dumetorum contains mannitol, saponins, coumarin glycosides, Leaves contain an iridoid-10- methylixoside. An iridoid glycoside, Ripe fruit contains glycosides, randioside A, mollisidial triterpenoid glycosides and randianin, six saponins-dumetoronins A to F. It cures abscess, ulcers, inflammation, wounds, tumours, skin diseases and have antibacterial activity. The pulp of fruit is believed by many practitioners to also have anthelmintic properties, and also used as an abortiffcient as folklore remedy. Various phytopharmacological evaluations have been reported in this literature for the important potential of the Randia dumetorum.
ABSTRACT
A case of amoeboma of the ascending colon with multiple amoebic liver abscess is being presented; which was mistakenly diagnosed as carcinoma of ascending colon with multiple secondaries in liver. Awareness of this previously unreported association is important because it adds to our knowledge of the spectrum of intestinal amoebiasis.